1TV2

Crystal structure of the hydroxylamine MtmB complex

1TV2 の概要

• エントリーDOI: 10.2210/pdb1tv2/pdb
• 関連するPDBエントリー: 1L2Q 1NTH
• 分子名称: Monomethylamine methyltransferase mtmB1, 5-HYDROXYAMINO-3-METHYL-PYRROLIDINE-2-CARBOXYLIC ACID (3 entities in total)
• 機能のキーワード: tim barrel, transferase
• 由来する生物種: Methanosarcina barkeri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50343.29

構造登録者

Hao, B.,Zhao, G.,Kang, P.T.,Soares, J.A.,Ferguson, T.K.,Gallucci, J.,Krzycki, J.A.,Chan, M.K. (登録日: 2004-06-26, 公開日: 2004-10-19, 最終更新日: 2024-10-30)

主引用文献

Hao, B.,Zhao, G.,Kang, P.T.,Soares, J.A.,Ferguson, T.K.,Gallucci, J.,Krzycki, J.A.,Chan, M.K.
Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid
Chem.Biol., 11:1317-1324, 2004

PubMed Abstract: L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB). To confirm L-pyrrolysine's identity, structures of MtmB have been determined following treatment with hydroxylamine, N-methylhydroxylamine, or dithionite. Analysis of these structures has provided additional support for the presence of the pyrroline ring and, together with previous mass spectroscopy data, has led us to assign the C(4)-substituent to a methyl group. Based on this assignment, synthetic L-pyrrolysine was prepared by chemical methods. Detailed study of this chemically synthesized L-pyrrolysine has allowed us to characterize its physical properties, to study its chemical stability, and to elucidate the role of its C(4) substituent. Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins.

PubMed: 15380192
DOI: 10.1016/j.chembiol.2004.07.011

実験手法

X-RAY DIFFRACTION (2 Å)