1TRO

CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION

1TRO の概要

• エントリーDOI: 10.2210/pdb1tro/pdb
• 分子名称: DNA (5'-D(*TP*GP*TP*AP*CP*TP*AP*GP*TP*TP*AP*AP*CP*TP*AP*GP*T P*AP*C)-3'), PROTEIN (TRP REPRESSOR), TRYPTOPHAN, ... (4 entities in total)
• 機能のキーワード: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli str. K12 substr.
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 73576.54

構造登録者

Otwinowski, Z.,Schevitz, R.W.,Zhang, R.-G.,Lawson, C.L.,Joachimiak, A.,Marmorstein, R.,Luisi, B.F.,Sigler, P.B. (登録日: 1992-08-30, 公開日: 1994-01-31, 最終更新日: 2024-02-14)

主引用文献

Otwinowski, Z.,Schevitz, R.W.,Zhang, R.G.,Lawson, C.L.,Joachimiak, A.,Marmorstein, R.Q.,Luisi, B.F.,Sigler, P.B.
Crystal structure of trp repressor/operator complex at atomic resolution.
Nature, 335:321-329, 1988

PubMed Abstract: The crystal structure of the trp repressor/operator complex shows an extensive contact surface, including 24 direct and 6 solvent-mediated hydrogen bonds to the phosphate groups of the DNA. There are no direct hydrogen bonds or non-polar contacts to the bases that can explain the repressor's specificity for the operator sequence. Rather, the sequence seems to be recognized indirectly through its effects on the geometry of the phosphate backbone, which in turn permits the formation of a stable interface. Water-mediated polar contacts to the bases also appear to contribute part of the specificity.

PubMed: 3419502
DOI: 10.1038/335321a0

実験手法

X-RAY DIFFRACTION (1.9 Å)