1TRI

THE CRYSTAL STRUCTURE OF AN ENGINEERED MONOMERIC TRIOSEPHOSPHATE ISOMERASE, MONOTIM: THE CORRECT MODELLING OF AN EIGHT-RESIDUE LOOP

1TRI の概要

• エントリーDOI: 10.2210/pdb1tri/pdb
• 分子名称: TRIOSEPHOSPHATE ISOMERASE, SULFATE ION (3 entities in total)
• 機能のキーワード: isomerase(intramolecular oxidoreductase)
• 由来する生物種: Trypanosoma brucei brucei
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26188.90

構造登録者

Wierenga, R.K. (登録日: 1993-10-08, 公開日: 1994-07-31, 最終更新日: 2024-02-14)

主引用文献

Borchert, T.V.,Abagyan, R.,Kishan, K.V.,Zeelen, J.P.,Wierenga, R.K.
The crystal structure of an engineered monomeric triosephosphate isomerase, monoTIM: the correct modelling of an eight-residue loop.
Structure, 1:205-213, 1993

PubMed Abstract: The triosephosphate isomerase (TIM) fold is found in several different classes of enzymes, most of which are oligomers; TIM itself always functions as a very tight dimer. It has recently been shown that a monomeric form of TIM ('monoTIM') can be constructed by replacing a 15-residue interface loop, loop-3, with an eight-residue fragment; modelling suggests that this should result in a short strain-free turn, resulting in the subsequent helix, helix-A3, having an additional turn at its amino terminus.

PubMed: 16100954
DOI: 10.1016/0969-2126(93)90021-8

実験手法

X-RAY DIFFRACTION (2.4 Å)