1TQY

The Actinorhodin Ketosynthase/Chain Length Factor

1TQY の概要

• エントリーDOI: 10.2210/pdb1tqy/pdb
• 分子名称: Actinorhodin polyketide putative beta-ketoacyl synthase 1, Actinorhodin polyketide putative beta-ketoacyl synthase 2, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: alpha-beta-alpha-beta-alpha, heterodimer, transferase
• 由来する生物種: Streptomyces coelicolor A3(2); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 355180.97

構造登録者

Keatinge-Clay, A.T.,Maltby, D.A.,Medzihradszky, K.F.,Khosla, C.,Stroud, R.M. (登録日: 2004-06-18, 公開日: 2004-07-27, 最終更新日: 2023-08-23)

主引用文献

Keatinge-Clay, A.T.,Maltby, D.A.,Medzihradszky, K.F.,Khosla, C.,Stroud, R.M.
An antibiotic factory caught in action.
Nat.Struct.Mol.Biol., 11:888-893, 2004

PubMed Abstract: The synthesis of aromatic polyketides, such as actinorhodin, tetracycline and doxorubicin, begins with the formation of a polyketide chain. In type II polyketide synthases (PKSs), chains are polymerized by the heterodimeric ketosynthase-chain length factor (KS-CLF). Here we present the 2.0-A structure of the actinorhodin KS-CLF, which shows polyketides being elongated inside an amphipathic tunnel approximately 17 A in length at the heterodimer interface. The structure resolves many of the questions about the roles of KS and CLF. Although CLF regulates chain length, it does not have an active site; KS must catalyze both chain initiation and elongation. We provide evidence that the first cyclization of the polyketide occurs within the KS-CLF tunnel. The mechanistic details of this central PKS polymerase could guide biosynthetic chemists in designing new pharmaceuticals and polymers.

PubMed: 15286722
DOI: 10.1038/nsmb808

実験手法

X-RAY DIFFRACTION (2 Å)