1TLY

Tsx structure

1TLY の概要

• エントリーDOI: 10.2210/pdb1tly/pdb
• 関連するPDBエントリー: 1tlw 1tlz
• 分子名称: Nucleoside-specific channel-forming protein tsx (1 entity in total)
• 機能のキーワード: nucleoside transporter, beta barrel, membrane protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell outer membrane; Multi-pass membrane protein: P0A927
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 64538.24

構造登録者

Ye, J.,van den Berg, B. (登録日: 2004-06-10, 公開日: 2004-08-24, 最終更新日: 2024-02-14)

主引用文献

Ye, J.,Van Den Berg, B.
Crystal structure of the bacterial nucleoside transporter Tsx.
Embo J., 23:3187-3195, 2004

PubMed Abstract: Tsx is a nucleoside-specific outer membrane (OM) transporter of Gram-negative bacteria. We present crystal structures of Escherichia coli Tsx in the absence and presence of nucleosides. These structures provide a mechanism for nucleoside transport across the bacterial OM. Tsx forms a monomeric, 12-stranded beta-barrel with a long and narrow channel spanning the outer membrane. The channel, which is shaped like a keyhole, contains several distinct nucleoside-binding sites, two of which are well defined. The base moiety of the nucleoside is located in the narrow part of the keyhole, while the sugar occupies the wider opening. Pairs of aromatic residues and flanking ionizable residues are involved in nucleoside binding. Nucleoside transport presumably occurs by diffusion from one binding site to the next.

PubMed: 15272310
DOI: 10.1038/sj.emboj.7600330

実験手法

X-RAY DIFFRACTION (3.01 Å)