1TLR

SOLUTION STRUCTURE OF TETRALOOP RECEPTOR RNA, NMR, 20 STRUCTURES

1TLR の概要

• エントリーDOI: 10.2210/pdb1tlr/pdb
• 分子名称: RNA TETRALOOP RECEPTOR (5'-R(GGCCUAAGACUUCGGUUAUGGCC)-3') (1 entity in total)
• 機能のキーワード: ribonucleic acid, tetraloop receptor, group i intron, group ii intron, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7356.39

構造登録者

Butcher, S.E.,Dieckmann, T.,Feigon, J. (登録日: 1997-09-11, 公開日: 1997-11-12, 最終更新日: 2024-05-22)

主引用文献

Butcher, S.E.,Dieckmann, T.,Feigon, J.
Solution structure of a GAAA tetraloop receptor RNA.
EMBO J., 16:7490-7499, 1997

PubMed Abstract: The GAAA tetraloop receptor is an 11-nucleotide RNA sequence that participates in the tertiary folding of a variety of large catalytic RNAs by providing a specific binding site for GAAA tetraloops. Here we report the solution structure of the isolated tetraloop receptor as solved by multidimensional, heteronuclear magnetic resonance spectroscopy. The internal loop of the tetraloop receptor has three adenosines stacked in a cross-strand or zipper-like fashion. This arrangement produces a high degree of base stacking within the asymmetric internal loop without extrahelical bases or kinking the helix. Additional interactions within the internal loop include a U. U mismatch pair and a G.U wobble pair. A comparison with the crystal structure of the receptor RNA bound to its tetraloop shows that a conformational change has to occur upon tetraloop binding, which is in good agreement with previous biochemical data. A model for an alternative binding site within the receptor is proposed based on the NMR structure, phylogenetic data and previous crystallographic structures of tetraloop interactions.

PubMed: 9405377
DOI: 10.1093/emboj/16.24.7490

実験手法

SOLUTION NMR