1TKO

Iron-oxo clusters biomineralizing on protein surfaces. Structural analysis of H.salinarum DpsA in its low and high iron states

1TKO の概要

• エントリーDOI: 10.2210/pdb1tko/pdb
• 関連するPDBエントリー: 1MOJ 1TJO 1TK6 1TKP
• 分子名称: Iron-rich dpsA-homolog protein, SULFATE ION, FE (III) ION, ... (5 entities in total)
• 機能のキーワード: dpsa, high iron, iron cluster, metal binding protein
• 由来する生物種: Halobacterium salinarum
• 細胞内の位置: Cytoplasm : Q9HMP7
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 82847.60

構造登録者

Zeth, K.,Offermann, S.,Essen, L.O.,Oesterhelt, D. (登録日: 2004-06-09, 公開日: 2004-10-19, 最終更新日: 2023-08-23)

主引用文献

Zeth, K.,Offermann, S.,Essen, L.O.,Oesterhelt, D.
Iron-oxo clusters biomineralizing on protein surfaces: structural analysis of Halobacterium salinarum DpsA in its low- and high-iron states.
Proc.Natl.Acad.Sci.USA, 101:13780-13785, 2004

PubMed Abstract: The crystal structure of the Dps-like (Dps, DNA-protecting protein during starvation) ferritin protein DpsA from the halophile Halobacterium salinarum was determined with low endogenous iron content at 1.6-A resolution. The mechanism of iron uptake and storage was analyzed in this noncanonical ferritin by three high-resolution structures at successively increasing iron contents. In the high-iron state of the DpsA protein, up to 110 iron atoms were localized in the dodecameric protein complex. For ultimate iron storage, the archaeal ferritin shell comprises iron-binding sites for iron translocation, oxidation, and nucleation. Initial iron-protein interactions occur through acidic residues exposed along the outer surface in proximity to the iron entry pore. This narrow pore permits translocation of ions toward the ferroxidase centers via two discrete steps. Iron oxidation proceeds by transient formation of tri-iron ferroxidase centers. Iron storage by biomineralization inside the ferritin shell occurs at two iron nucleation centers. Here, a single iron atom provides a structural seed for iron-oxide cluster formation. The clusters with up to five iron atoms adopt a geometry that is different from natural biominerals like magnetite but resembles iron clusters so far known only from bioinorganic model compounds.

PubMed: 15365182
DOI: 10.1073/pnas.0401821101

実験手法

X-RAY DIFFRACTION (2.9 Å)