1TIG

TRANSLATION INITIATION FACTOR 3 C-TERMINAL DOMAIN

Summary for 1TIG

• Entry DOI: 10.2210/pdb1tig/pdb
• Related: 1TIF
• Descriptor: TRANSLATION INITIATION FACTOR 3 (2 entities in total)
• Functional Keywords: if3 c-terminal domain, ribosome binding factor
• Biological source: Geobacillus stearothermophilus
• Cellular location: Cytoplasm: P03000
• Total number of polymer chains: 1
• Total formula weight: 10752.57

Authors

Biou, V.,Shu, F.,Ramakrishnan, V. (deposition date: 1995-08-16, release date: 1995-12-07, Last modification date: 2024-02-14)

Primary citation

Biou, V.,Shu, F.,Ramakrishnan, V.
X-ray crystallography shows that translational initiation factor IF3 consists of two compact alpha/beta domains linked by an alpha-helix.
EMBO J., 14:4056-4064, 1995

PubMed Abstract: The structures of the two domains of translational initiation factor IF3 from Bacillus stearothermophilus have been solved by X-ray crystallography using single wavelength anomalous scattering and multiwavelength anomalous diffraction. Each of the two domains has an alpha/beta topology, with an exposed beta-sheet that is reminiscent of several ribosomal and other RNA binding proteins. An alpha-helix that protrudes out from the body of the N-terminal domain towards the C-terminal domain suggests that IF3 consists of two RNA binding domains connected by an alpha-helix and that it may bridge two regions of the ribosome. This represents the first high resolution structural information on a translational initiation factor.

PubMed: 7664745

Experimental method

X-RAY DIFFRACTION (2 Å)