1TC3

TRANSPOSASE TC3A1-65 FROM CAENORHABDITIS ELEGANS

1TC3 の概要

• エントリーDOI: 10.2210/pdb1tc3/pdb
• 分子名称: DNA (5'-D(*AP*GP*GP*GP*GP*GP*GP*GP*TP*CP*CP*TP*AP*TP*AP*GP*A P*AP*CP*TP*T)-3'), DNA (5'-D(*AP*GP*TP*TP*CP*TP*AP*TP*AP*GP*GP*AP*CP*CP*CP*CP*C P*CP*CP*T)-3'), PROTEIN (TC3 TRANSPOSASE), ... (4 entities in total)
• 機能のキーワード: transposase, dna binding, helix-turn-helix, tc1/mariner family, complex (transposase-dna), dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Caenorhabditis elegans
• 細胞内の位置: Nucleus: P34257
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 18386.86

構造登録者

Van Pouderoyen, G.,Ketting, R.F.,Perrakis, A.,Plasterk, R.H.A.,Sixma, T.K. (登録日: 1997-07-07, 公開日: 1997-11-21, 最終更新日: 2024-02-14)

主引用文献

van Pouderoyen, G.,Ketting, R.F.,Perrakis, A.,Plasterk, R.H.,Sixma, T.K.
Crystal structure of the specific DNA-binding domain of Tc3 transposase of C.elegans in complex with transposon DNA.
EMBO J., 16:6044-6054, 1997

PubMed Abstract: The crystal structure of the complex between the N-terminal DNA-binding domain of Tc3 transposase and an oligomer of transposon DNA has been determined. The specific DNA-binding domain contains three alpha-helices, of which two form a helix-turn-helix (HTH) motif. The recognition of transposon DNA by the transposase is mediated through base-specific contacts and complementarity between protein and sequence-dependent deformations of the DNA. The HTH motif makes four base-specific contacts with the major groove, and the N-terminus makes three base-specific contacts with the minor groove. The DNA oligomer adopts a non-linear B-DNA conformation, made possible by a stretch of seven G:C base pairs at one end and a TATA sequence towards the other end. Extensive contacts (seven salt bridges and 16 hydrogen bonds) of the protein with the DNA backbone allow the protein to probe and recognize the sequence-dependent DNA deformation. The DNA-binding domain forms a dimer in the crystals. Each monomer binds a separate transposon end, implying that the dimer plays a role in synapsis, necessary for the simultaneous cleavage of both transposon termini.

PubMed: 9312061
DOI: 10.1093/emboj/16.19.6044

実験手法

X-RAY DIFFRACTION (2.45 Å)