1T5W

HLA-DR1 in complex with a synthetic peptide (AAYSDQATPLLLSPR)

1T5W の概要

• エントリーDOI: 10.2210/pdb1t5w/pdb
• 関連するPDBエントリー: 1AQD 1DLH 1T5X
• 分子名称: HLA class II histocompatibility antigen, DR alpha chain, HLA class II histocompatibility antigen, DRB1-1 beta chain, 15-mer peptide fragment of Regulatory protein MIG1, ... (4 entities in total)
• 機能のキーワード: mhc class ii; najor histocompatibility complex protein; hla-dr1; antigen; peptide, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein: P01903 P04229; Nucleus: P27705
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 89312.26

構造登録者

Zavala-Ruiz, Z.,Strug, I.,Anderson, M.W.,Gorski, J.,Stern, L.J. (登録日: 2004-05-05, 公開日: 2004-08-17, 最終更新日: 2024-10-30)

主引用文献

Zavala-Ruiz, Z.,Strug, I.,Anderson, M.W.,Gorski, J.,Stern, L.J.
A Polymorphic Pocket at the P10 Position Contributes to Peptide Binding Specificity in Class II MHC Proteins
Chem.Biol., 11:1395-1402, 2004

PubMed Abstract: Peptides bind to class II major histocompatibility complex (MHC) proteins in an extended conformation. Pockets in the peptide binding site spaced to accommodate peptide side chains at the P1, P4, P6, and P9 positions have been previously characterized and help to explain the obtained peptide binding specificity. However, two peptides differing only at P10 have significantly different binding affinities for HLA-DR1. The structure of HLA-DR1 in complex with the tighter binding peptide shows that the peptide binds in the usual polyproline type II conformation, but with the P10 residue accommodated in a shallow pocket at the end of the binding groove. HLA-DR1 variants with polymorphic residues at these positions were produced and found to exhibit different side chain specificity at the P10 position. These results define a new specificity position in HLA-DR proteins.

PubMed: 15489166
DOI: 10.1016/j.chembiol.2004.08.007

実験手法

X-RAY DIFFRACTION (2.4 Å)