1T5R

STRUCTURE OF THE PANTON-VALENTINE LEUCOCIDIN S COMPONENT FROM STAPHYLOCOCCUS AUREUS

Summary for 1T5R

• Entry DOI: 10.2210/pdb1t5r/pdb
• Related: 1PVL
• Descriptor: LukS-PV (2 entities in total)
• Functional Keywords: bi-component leucotoxin, pore-forming toxin, staphylococcus aureus, s component leucocidin, unknown function
• Biological source: Staphylococcus phage PVL
• Total number of polymer chains: 8
• Total formula weight: 258844.97

Authors

Guillet, V.,Roblin, P.,Keller, D.,Prevost, G.,Mourey, L. (deposition date: 2004-05-05, release date: 2004-08-24, Last modification date: 2023-08-23)

Primary citation

Guillet, V.,Roblin, P.,Werner, S.,Coraiola, M.,Menestrina, G.,Monteil, H.,Mourey, L.
Crystal structure of leucotoxin S component: new insight into the Staphylococcal beta-barrel pore-forming toxins.
J.Biol.Chem., 279:41028-41037, 2004

PubMed Abstract: Staphylococcal leucocidins and gamma-hemolysins (leucotoxins) are bi-component toxins that form lytic transmembrane pores. Their cytotoxic activities require the synergistic association of a class S component and a class F component, produced as water-soluble monomers that form hetero-oligomeric membrane-associated complexes. Strains that produce the Panton-Valentine leucocidin are clinically associated with cutaneous lesions and community-acquired pneumonia. In a previous study, we determined the crystal structure of the F monomer from the Panton-Valentine leucocidin. To derive information on the second component of the leucotoxins, the x-ray structure of the S protein from the Panton-Valentine leucocidin was solved to 2.0 angstrom resolution using a tetragonal crystal form that contains eight molecules in the asymmetric unit. The structure demonstrates the different conformation of the domain involved in membrane contacts and illustrates sequence and tertiary structure variabilities of the pore-forming leucotoxins. Mutagenesis studies at a key surface residue (Thr-28) further support the important role played by these microheterogeneities for the assembly of the bipartite leucotoxins.

PubMed: 15262988
DOI: 10.1074/jbc.M406904200

Experimental method

X-RAY DIFFRACTION (2 Å)