1T2S

Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain

1T2S の概要

• エントリーDOI: 10.2210/pdb1t2s/pdb
• 関連するPDBエントリー: 1t2s 1UPO
• 分子名称: 5'-D(*CP*TP*CP*AP*C)-3', Argonaute 2 (2 entities in total)
• 機能のキーワード: nucleic acid binding protein, dna, nucleic acid binding protein-dna complex, nucleic acid binding protein/dna
• 由来する生物種: Drosophila melanogaster (fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15368.96

構造登録者

Lingel, A.,Simon, B.,Izaurralde, E.,Sattler, M. (登録日: 2004-04-22, 公開日: 2004-06-01, 最終更新日: 2024-05-22)

主引用文献

Lingel, A.,Simon, B.,Izaurralde, E.,Sattler, M.
Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain.
Nat.Struct.Mol.Biol., 11:576-577, 2004

PubMed Abstract: We describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides. The structures reveal a unique mode of single-stranded nucleic acid binding in which the two 3'-terminal nucleotides are buried in a hydrophobic cleft. We propose that the PAZ domain contributes to the specific recognition of siRNAs by providing a binding pocket for their characteristic two-nucleotide 3' overhangs.

PubMed: 15156196
DOI: 10.1038/nsmb777

実験手法

SOLUTION NMR