1T2R

Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain

Summary for 1T2R

• Entry DOI: 10.2210/pdb1t2r/pdb
• Related: 1UPO 1t2s
• Descriptor: 5'-R(*CP*UP*CP*AP*C)-3', Argonaute 2 (2 entities in total)
• Functional Keywords: nucleic acid binding protein, rna, nucleic acid binding protein-rna complex, nucleic acid binding protein/rna
• Biological source: Drosophila melanogaster (fruit fly)
• Total number of polymer chains: 2
• Total formula weight: 15434.93

Authors

Lingel, A.,Simon, B.,Izaurralde, E.,Sattler, M. (deposition date: 2004-04-22, release date: 2004-06-01, Last modification date: 2024-05-22)

Primary citation

Lingel, A.,Simon, B.,Izaurralde, E.,Sattler, M.
Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain.
Nat.Struct.Mol.Biol., 11:576-577, 2004

PubMed Abstract: We describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides. The structures reveal a unique mode of single-stranded nucleic acid binding in which the two 3'-terminal nucleotides are buried in a hydrophobic cleft. We propose that the PAZ domain contributes to the specific recognition of siRNAs by providing a binding pocket for their characteristic two-nucleotide 3' overhangs.

PubMed: 15156196
DOI: 10.1038/nsmb777

Experimental method

SOLUTION NMR