1SWU

STREPTAVIDIN MUTANT Y43F

1SWU の概要

• エントリーDOI: 10.2210/pdb1swu/pdb
• 分子名称: STREPTAVIDIN, (4R)-2-METHYLPENTANE-2,4-DIOL, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• 機能のキーワード: biotin binding protein, protein binding
• 由来する生物種: Streptomyces avidinii
• 細胞内の位置: Secreted: P22629
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 53770.39

構造登録者

Freitag, S.,Le Trong, I.,Klumb, L.A.,Stayton, P.S.,Stenkamp, R.E. (登録日: 1998-10-12, 公開日: 1999-11-10, 最終更新日: 2024-12-25)

主引用文献

Freitag, S.,Le Trong, I.,Klumb, L.A.,Stayton, P.S.,Stenkamp, R.E.
Atomic resolution structure of biotin-free Tyr43Phe streptavidin: what is in the binding site?
Acta Crystallogr.,Sect.D, 55:1118-1126, 1999

PubMed Abstract: The streptavidin-biotin system is an example of a high-affinity protein-ligand pair (Ka approximately 10(13) mol-1). The thermodynamic and structural properties have been extensively studied as a model system for protein-ligand interactions. Here, the X-ray crystal structure of a streptavidin mutant of a residue hydrogen bonding to biotin [Tyr43Phe (Y43F)] is reported at atomic resolution (1.14 A). The biotin-free structure was refined with anisotropic displacement parameters (SHELXL97 program package). The high-resolution data also allowed interpretation of side-chain and residue disorder in 41 residues where alternate conformations were refined. The Y43F mutation is unambiguously observed in difference maps, although only a single O atom per monomer is altered. The atomic resolution enabled the identification of 2-methyl-2, 4-pentanediol (MPD) molecules in the biotin-binding pocket for the first time. Electron density for MPD was observed in all four subunit binding sites of the tetrameric protein. This was not possible with data at lower resolution (1.8-2.3 A) for wild-type streptavidin or mutants in the same crystal form using MPD in the crystallization. The impact of MPD binding on these studies is discussed.

PubMed: 10329773
DOI: 10.1107/S0907444999002322

実験手法

X-RAY DIFFRACTION (1.14 Å)