1SUM

Crystal structure of a hypothetical protein at 2.0 A resolution

1SUM の概要

• エントリーDOI: 10.2210/pdb1sum/pdb
• 分子名称: Phosphate transport system protein phoU homolog 2, FE (III) ION, NICKEL (II) ION, ... (6 entities in total)
• 機能のキーワード: phou, abc transport, pst, structural genomics, berkeley structural genomics center, bsgc, structure funded by nih, protein structure initiative, psi, transport protein
• 由来する生物種: Thermotoga maritima
• 細胞内の位置: Cytoplasm : Q9X256
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27498.82

構造登録者

Liu, J.,Lou, Y.,Yokota, H.,Adams, P.D.,Kim, R.,Kim, S.H.,Berkeley Structural Genomics Center (BSGC) (登録日: 2004-03-26, 公開日: 2004-08-24, 最終更新日: 2017-10-11)

主引用文献

Liu, J.,Lou, Y.,Yokota, H.,Adams, P.D.,Kim, R.,Kim, S.H.
Crystal structure of a PhoU protein homologue: a new class of metalloprotein containing multinuclear iron clusters.
J.Biol.Chem., 280:15960-15966, 2005

PubMed Abstract: PhoU proteins are known to play a role in the regulation of phosphate uptake. In Thermotoga maritima, two PhoU homologues have been identified bioinformatically. Here we report the crystal structure of one of the PhoU homologues at 2.0 A resolution. The structure of the PhoU protein homologue contains a highly symmetric new structural fold composed of two repeats of a three-helix bundle. The structure unexpectedly revealed a trinuclear and a tetranuclear iron cluster that were found to be bound on the surface. Each of the two multinuclear iron clusters is coordinated by a conserved E(D)XXXD motif pair. Our structure reveals a new class of metalloprotein containing multinuclear iron clusters. The possible functional implication based on the structure are discussed.

PubMed: 15716271
DOI: 10.1074/jbc.M414117200

実験手法

X-RAY DIFFRACTION (2 Å)