1SU4

Crystal structure of calcium ATPase with two bound calcium ions

「1EUL」から置き換えられました

1SU4 の概要

• エントリーDOI: 10.2210/pdb1su4/pdb
• 関連するPDBエントリー: 1IWO 1VFP
• NMR情報: BMRB: 5765
• 分子名称: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, CALCIUM ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: ion pump, membrane protein, p-type atpase, active transport, hydrolase
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• 細胞内の位置: Endoplasmic reticulum membrane ; Multi-pass membrane protein : P04191
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 109705.72

構造登録者

Toyoshima, C.,Nakasako, M.,Nomura, H.,Ogawa, H. (登録日: 2004-03-26, 公開日: 2004-05-04, 最終更新日: 2024-10-23)

主引用文献

Toyoshima, C.,Nakasako, M.,Nomura, H.,Ogawa, H.
Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution
Nature, 405:647-655, 2000

PubMed Abstract: Calcium ATPase is a member of the P-type ATPases that transport ions across the membrane against a concentration gradient. Here we have solved the crystal structure of the calcium ATPase of skeletal muscle sarcoplasmic reticulum (SERCA1a) at 2.6 A resolution with two calcium ions bound in the transmembrane domain, which comprises ten alpha-helices. The two calcium ions are located side by side and are surrounded by four transmembrane helices, two of which are unwound for efficient coordination geometry. The cytoplasmic region consists of three well separated domains, with the phosphorylation site in the central catalytic domain and the adenosine-binding site on another domain. The phosphorylation domain has the same fold as haloacid dehalogenase. Comparison with a low-resolution electron density map of the enzyme in the absence of calcium and with biochemical data suggests that large domain movements take place during active transport.

PubMed: 10864315
DOI: 10.1038/35015017

実験手法

X-RAY DIFFRACTION (2.4 Å)