1SQ9

Structure of Ski8p, a WD repeat protein involved in mRNA degradation and meiotic recombination

1SQ9 の概要

• エントリーDOI: 10.2210/pdb1sq9/pdb
• 分子名称: Antiviral protein SKI8, SULFATE ION (3 entities in total)
• 機能のキーワード: wd repeat, beta-transducin repeat, wd40 repeat, beta propeller, antiviral protein, recombination
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Cytoplasm: Q02793
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44475.65

構造登録者

Madrona, A.Y.,Wilson, D.K. (登録日: 2004-03-18, 公開日: 2004-05-25, 最終更新日: 2024-02-14)

主引用文献

Madrona, A.Y.,Wilson, D.K.
The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure.
Protein Sci., 13:1557-1565, 2004

PubMed Abstract: Ski8p is a 44-kD protein that primarily functions in the regulation of exosome-mediated, 3'--> 5' degradation of damaged mRNA. It does so by forming a complex with two partner proteins, Ski2p and Ski3p, which complete a complex that is capable of recruiting and activating the exosome/Ski7p complex that functions in RNA degradation. Ski8p also functions in meiotic recombination in complex with Spo11 in yeast. It is one of the many hundreds of primarily eukaryotic proteins containing tandem copies of WD repeats (also known as WD40 or beta-transducin repeats), which are short ~40 amino acid motifs, often terminating in a Trp-Asp dipeptide. Genomic analyses have demonstrated that WD repeats are found in 1%-2% of proteins in a typical eukaryote, but are extremely rare in prokaryotes. Almost all structurally characterized WD-repeat proteins are composed of seven such repeats and fold into seven-bladed beta propellers. Ski8p was thought to contain five WD repeats on the basis of primary sequence analysis implying a five-bladed propeller. The 1.9 A crystal structure unexpectedly exhibits a seven-bladed propeller fold with seven structurally authentic WD repeats. Structure-based sequence alignments show additional sequence diversity in the two undetected repeats. This demonstrates that many WD repeats have not yet been identified in sequences and also raises the possibility that the seven-bladed propeller may be the predominant fold for this family of proteins.

PubMed: 15152089
DOI: 10.1110/ps.04704704

実験手法

X-RAY DIFFRACTION (1.9 Å)