1SQ2

Crystal Structure Analysis of the Nurse Shark New Antigen Receptor (NAR) Variable Domain in Complex With Lysozyme

1SQ2 の概要

• エントリーDOI: 10.2210/pdb1sq2/pdb
• 分子名称: Lysozyme C, novel antigen receptor, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: immunoglobulin fold, protein-protein complex, hydrolase-immune system complex, hydrolase/immune system
• 由来する生物種: Ginglymostoma cirratum (nurse shark); 詳細
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26732.20

構造登録者

Stanfield, R.L.,Dooley, H.,Flajnik, M.F.,Wilson, I.A. (登録日: 2004-03-17, 公開日: 2004-08-24, 最終更新日: 2024-10-09)

主引用文献

Stanfield, R.L.,Dooley, H.,Flajnik, M.F.,Wilson, I.A.
Crystal structure of a shark single-domain antibody V region in complex with lysozyme.
Science, 305:1770-1773, 2004

PubMed Abstract: Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies.

PubMed: 15319492
DOI: 10.1126/science.1101148

実験手法

X-RAY DIFFRACTION (1.45 Å)