1SNY

Carbonyl reductase Sniffer of D. melanogaster

1SNY の概要

• エントリーDOI: 10.2210/pdb1sny/pdb
• 分子名称: sniffer CG10964-PA, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (3 entities in total)
• 機能のキーワード: alpha and beta protein, rossmann fold, dinucleotide binding motif, oxidoreductase
• 由来する生物種: Drosophila melanogaster (fruit fly)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29783.55

構造登録者

Sgraja, T.,Ulschmid, J.,Becker, K.,Schneuwly, S.,Klebe, G.,Reuter, K.,Heine, A. (登録日: 2004-03-12, 公開日: 2004-09-28, 最終更新日: 2011-07-13)

主引用文献

Sgraja, T.,Ulschmid, J.,Becker, K.,Schneuwly, S.,Klebe, G.,Reuter, K.,Heine, A.
Structural Insights into the Neuroprotective-acting Carbonyl Reductase Sniffer of Drosophila melanogaster.
J.Mol.Biol., 342:1613-1624, 2004

PubMed Abstract: In vivo studies with the fruit-fly Drosophila melanogaster have shown that the Sniffer protein prevents age-dependent and oxidative stress-induced neurodegenerative processes. Sniffer is a NADPH-dependent carbonyl reductase belonging to the enzyme family of short-chain dehydrogenases/reductases (SDRs). The crystal structure of the homodimeric Sniffer protein from Drosophila melanogaster in complex with NADP+ has been determined by multiple-wavelength anomalous dispersion and refined to a resolution of 1.75 A. The observed fold represents a typical dinucleotide-binding domain as detected for other SDRs. With respect to the cofactor-binding site and the region referred to as substrate-binding loop, the Sniffer protein shows a striking similarity to the porcine carbonyl reductase (PTCR). This loop, in both Sniffer and PTCR, is substantially shortened compared to other SDRs. In most enzymes of the SDR family this loop adopts a well-defined conformation only after substrate binding and remains disordered in the absence of any bound ligands or even if only the dinucleotide cofactor is bound. In the structure of the Sniffer protein, however, the conformation of this loop is well defined, although no substrate is present. Molecular modeling studies provide an idea of how binding of substrate molecules to Sniffer could possibly occur.

PubMed: 15364585
DOI: 10.1016/j.jmb.2004.08.020

実験手法

X-RAY DIFFRACTION (1.75 Å)