1SKY

CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3

1SKY の概要

• エントリーDOI: 10.2210/pdb1sky/pdb
• 分子名称: F1-ATPASE, SULFATE ION (3 entities in total)
• 機能のキーワード: atp synthase, f1fo atp synthase, f1-atpase, alpha3beta3 subcomplex of f1-atpase, hydrolase
• 由来する生物種: Bacillus sp.; 詳細
• 細胞内の位置: Cell membrane; Peripheral membrane protein (By similarity): P09219 P07677
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 106838.54

構造登録者

Shirakihara, Y.,Leslie, A.G.W.,Abrahams, J.P.,Walker, J.E.,Ueda, T.,Sekimoto, Y.,Kambara, M.,Saika, K.,Kagawa, Y.,Yoshida, M. (登録日: 1997-02-26, 公開日: 1998-03-04, 最終更新日: 2024-02-14)

主引用文献

Shirakihara, Y.,Leslie, A.G.,Abrahams, J.P.,Walker, J.E.,Ueda, T.,Sekimoto, Y.,Kambara, M.,Saika, K.,Kagawa, Y.,Yoshida, M.
The crystal structure of the nucleotide-free alpha 3 beta 3 subcomplex of F1-ATPase from the thermophilic Bacillus PS3 is a symmetric trimer.
Structure, 5:825-836, 1997

PubMed Abstract: F1-ATPase, an oligomeric assembly with subunit stoichiometry alpha 3 beta 3 gamma delta epsilon, is the catalytic component of the ATP synthase complex, which plays a central role in energy transduction in bacteria, chloroplasts and mitochondria. The crystal structure of bovine mitochondrial F1-ATPase displays a marked asymmetry in the conformation and nucleotide content of the catalytic beta subunits. The alpha 3 beta 3 subcomplex of F1-ATPase has been assembled from subunits of the moderately thermophilic Bacillus PS3 made in Escherichia coli, and the subcomplex is active but does not show the catalytic cooperativity of intact F1-ATPase. The structure of this subcomplex should provide new information on the conformational variability of F1-ATPase and may provide insights into the unusual catalytic mechanism employed by this enzyme.

PubMed: 9261073
DOI: 10.1016/S0969-2126(97)00236-0

実験手法

X-RAY DIFFRACTION (3.2 Å)