1SJ9

Crystal structure of the uridine phosphorylase from Salmonella typhimurium at 2.5A resolution

1SJ9 の概要

• エントリーDOI: 10.2210/pdb1sj9/pdb
• 関連するPDBエントリー: 1K3F 1RYZ
• 分子名称: Uridine phosphorylase, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: nucleoside phosphorylase, transferase
• 由来する生物種: Salmonella typhimurium
• 細胞内の位置: Cytoplasm : P0A1F6
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 163204.49

構造登録者

Dontsova, M.,Gabdoulkhakov, A.,Morgunova, E.,Garber, M.,Nikonov, S.,Betzel, C.,Ealick, S.,Mikhailov, A. (登録日: 2004-03-03, 公開日: 2005-03-08, 最終更新日: 2023-08-23)

主引用文献

Dontsova, M.V.,Gabdoulkhakov, A.G.,Molchan, O.K.,Lashkov, A.A.,Garber, M.B.,Mironov, A.S.,Zhukhlistova, N.E.,Morgunova, E.Y.,Voelter, W.,Betzel, C.,Zhang, Y.,Ealick, S.E.,Mikhailov, A.M.
Preliminary investigation of the three-dimensional structure of Salmonella typhimurium uridine phosphorylase in the crystalline state.
Acta Crystallogr.,Sect.F, 61:337-340, 2005

PubMed Abstract: Uridine phosphorylase (UPh) catalyzes the phosphorolytic cleavage of the C-N glycosidic bond of uridine to ribose 1-phosphate and uracil in the pyrimidine-salvage pathway. The crystal structure of the Salmonella typhimurium uridine phosphorylase (StUPh) has been determined at 2.5 A resolution and refined to an R factor of 22.1% and an Rfree of 27.9%. The hexameric StUPh displays 32 point-group symmetry and utilizes both twofold and threefold non-crystallographic axes. A phosphate is bound at the active site and forms hydrogen bonds to Arg91, Arg30, Thr94 and Gly26 of one monomer and Arg48 of an adjacent monomer. The hexameric StUPh model reveals a close structural relationship to Escherichia coli uridine phosphorylase (EcUPh).

PubMed: 16511035
DOI: 10.1107/S1744309105007463

実験手法

X-RAY DIFFRACTION (2.5 Å)