1SIG

CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE

Summary for 1SIG

• Entry DOI: 10.2210/pdb1sig/pdb
• Descriptor: RNA POLYMERASE PRIMARY SIGMA FACTOR (2 entities in total)
• Functional Keywords: rna polymerase sigma factor, transcription regulation
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 39092.88

Authors

Malhotra, A.,Severinova, E.,Darst, S.A. (deposition date: 1997-02-18, release date: 1997-05-15, Last modification date: 2024-02-14)

Primary citation

Malhotra, A.,Severinova, E.,Darst, S.A.
Crystal structure of a sigma 70 subunit fragment from E. coli RNA polymerase.
Cell(Cambridge,Mass.), 87:127-136, 1996

PubMed Abstract: The 2.6 A crystal structure of a fragment of the sigma 70 promoter specificity subunit of E. coli RNA polymerase is described. Residues involved in core RNA polymerase binding lie on one face of the structure. On the opposite face, aligned along one helix, are exposed residues that interact with the -10 consensus promoter element (the Pribnow box), including four aromatic residues involved in promoter melting. The structure suggests one way in which DNA interactions may be inhibited in the absence of RNA polymerase and provides a framework for the interpretation of a large number of genetic and biochemical analyses.

PubMed: 8858155
DOI: 10.1016/S0092-8674(00)81329-X

Experimental method

X-RAY DIFFRACTION (2.6 Å)