1SIG

CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE

1SIG の概要

• エントリーDOI: 10.2210/pdb1sig/pdb
• 分子名称: RNA POLYMERASE PRIMARY SIGMA FACTOR (2 entities in total)
• 機能のキーワード: rna polymerase sigma factor, transcription regulation
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39092.88

構造登録者

Malhotra, A.,Severinova, E.,Darst, S.A. (登録日: 1997-02-18, 公開日: 1997-05-15, 最終更新日: 2024-02-14)

主引用文献

Malhotra, A.,Severinova, E.,Darst, S.A.
Crystal structure of a sigma 70 subunit fragment from E. coli RNA polymerase.
Cell(Cambridge,Mass.), 87:127-136, 1996

PubMed Abstract: The 2.6 A crystal structure of a fragment of the sigma 70 promoter specificity subunit of E. coli RNA polymerase is described. Residues involved in core RNA polymerase binding lie on one face of the structure. On the opposite face, aligned along one helix, are exposed residues that interact with the -10 consensus promoter element (the Pribnow box), including four aromatic residues involved in promoter melting. The structure suggests one way in which DNA interactions may be inhibited in the absence of RNA polymerase and provides a framework for the interpretation of a large number of genetic and biochemical analyses.

PubMed: 8858155
DOI: 10.1016/S0092-8674(00)81329-X

実験手法

X-RAY DIFFRACTION (2.6 Å)