1SHC

SHC PTB DOMAIN COMPLEXED WITH A TRKA RECEPTOR PHOSPHOPEPTIDE, NMR, MINIMIZED AVERAGE STRUCTURE

Summary for 1SHC

• Entry DOI: 10.2210/pdb1shc/pdb
• Descriptor: SHC, TRKA RECEPTOR PHOSPHOPEPTIDE (2 entities in total)
• Functional Keywords: complex (signal transduction-peptide), phosphotyrosine binding domain (ptb), complex (signal transduction-peptide) complex, complex (signal transduction/peptide)
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm. Isoform p46Shc: Mitochondrion matrix. Isoform p66Shc: Mitochondrion (By similarity): P29353; Cell membrane; Single-pass type I membrane protein (By similarity): P04629
• Total number of polymer chains: 2
• Total formula weight: 22924.09

Authors

Zhou, M.-M.,Ravichandran, K.S.,Olejniczak, E.T.,Petros, A.M.,Meadows, R.P.,Sattler, M.,Harlan, J.E.,Wade, W.S.,Burakoff, S.J.,Fesik, S.W. (deposition date: 1996-03-27, release date: 1997-05-15, Last modification date: 2024-10-30)

Primary citation

Zhou, M.M.,Ravichandran, K.S.,Olejniczak, E.F.,Petros, A.M.,Meadows, R.P.,Sattler, M.,Harlan, J.E.,Wade, W.S.,Burakoff, S.J.,Fesik, S.W.
Structure and ligand recognition of the phosphotyrosine binding domain of Shc.
Nature, 378:584-592, 1995

PubMed Abstract: The nuclear magnetic resonance structure of the phosphotyrosine binding (PTB) domain of Shc complexed to a phosphopeptide reveals an alternative means of recognizing tyrosine-phosphorylated proteins. Unlike in SH2 domains, the phosphopeptide forms an antiparallel beta-strand with a beta-sheet of the protein, interacts with a hydrophobic pocket through the (pY-5) residue, and adopts a beta-turn. The PTB domain is structurally similar to pleckstrin homology domains (a beta-sandwich capped by an alpha-helix) and binds to acidic phospholipids, suggesting a possible role in membrane localization.

PubMed: 8524391
DOI: 10.1038/378584a0

Experimental method

SOLUTION NMR