1SGV

STRUCTURE OF TRNA PSI55 PSEUDOURIDINE SYNTHASE (TRUB)

「1S71」から置き換えられました

1SGV の概要

• エントリーDOI: 10.2210/pdb1sgv/pdb
• 分子名称: tRNA pseudouridine synthase B (2 entities in total)
• 機能のキーワード: hinged motion, trna modification, structural genomics, psi, protein structure initiative, tb structural genomics consortium, tbsgc, lyase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 67721.72

構造登録者

Chaudhuri, B.N.,Chan, S.,Perry, L.J.,Yeates, T.O.,TB Structural Genomics Consortium (TBSGC) (登録日: 2004-02-24, 公開日: 2004-03-02, 最終更新日: 2023-08-23)

主引用文献

Chaudhuri, B.N.,Chan, S.,Perry, L.J.,Yeates, T.O.
Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft.
J.Biol.Chem., 279:24585-24591, 2004

PubMed Abstract: The three-dimensional structure of the RNA-modifying enzyme, psi55 tRNA pseudouridine synthase from Mycobacterium tuberculosis, is reported. The 1.9-A resolution crystal structure reveals the enzyme, free of substrate, in two distinct conformations. The structure depicts an interesting mode of protein flexibility involving a hinged bending in the central beta-sheet of the catalytic module. Key parts of the active site cleft are also found to be disordered in the substrate-free form of the enzyme. The hinge bending appears to act as a clamp to position the substrate. Our structural data furthers the previously proposed mechanism of tRNA recognition. The present crystal structure emphasizes the significant role that protein dynamics must play in tRNA recognition, base flipping, and modification.

PubMed: 15028724
DOI: 10.1074/jbc.M401045200

実験手法

X-RAY DIFFRACTION (1.9 Å)