1S9S

SOLUTION STRUCTURE OF MLV PSI SITE

1S9S の概要

• エントリーDOI: 10.2210/pdb1s9s/pdb
• 分子名称: MLV Psi encapsidation site (1 entity in total)
• 機能のキーワード: mlv, a-minor k-turn, stem loop, bulge, g-u mismatch, g-a mismatch, u-u mismatch, a-c mismatch, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32703.43

構造登録者

D'Souza, V.,Dey, A.,Habib, D.,Summers, M.F. (登録日: 2004-02-05, 公開日: 2004-04-06, 最終更新日: 2024-05-22)

主引用文献

D'Souza, V.,Dey, A.,Habib, D.,Summers, M.F.
NMR structure of the 101-nucleotide core encapsidation signal of the Moloney Murine Leukemia Virus.
J.Mol.Biol., 337:427-442, 2004

PubMed Abstract: The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly. We have determined the structure of a 101-nucleotide RNA that contains this signal (called mPsi) using a novel isotope-edited NMR approach. The method is robust and should be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide linker. The structure contains five mismatched base-pairs, an unusual C.CG base-triple platform, and a novel "A-minor K-turn," in which unpaired adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond with the phosphodiester of A341. Phylogenetic analyses indicate that these essential structural elements are conserved among the murine C-type retroviruses.

PubMed: 15003457
DOI: 10.1016/j.jmb.2004.01.037

実験手法

SOLUTION NMR