1S97

DPO4 with GT mismatch

1S97 の概要

• エントリーDOI: 10.2210/pdb1s97/pdb
• 分子名称: 5'-D(*GP*GP*GP*GP*GP*AP*AP*GP*GP*AP*CP*TP*G)-3', 5'-D(*T*TP*CP*AP*GP*TP*AP*GP*TP*CP*CP*TP*TP*CP*CP*CP*CP*C)-3', DNA polymerase IV, ... (6 entities in total)
• 機能のキーワード: protein-dna complex, dna duplex, g.t mismatch, transferase-dna complex, transferase/dna
• 由来する生物種: Sulfolobus solfataricus
• 細胞内の位置: Cytoplasm (Probable): Q97W02
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 200997.11

構造登録者

Trincao, J.,Johnson, R.E.,Wolfle, W.T.,Escalante, C.R.,Prakash, S.,Prakash, L.,Aggarwal, A.K. (登録日: 2004-02-03, 公開日: 2004-04-27, 最終更新日: 2023-08-23)

主引用文献

Trincao, J.,Johnson, R.E.,Wolfle, W.T.,Escalante, C.R.,Prakash, S.,Prakash, L.,Aggarwal, A.K.
Dpo4 is hindered in extending a G.T mismatch by a reverse wobble
Nat.Struct.Mol.Biol., 11:457-462, 2004

PubMed Abstract: The ability or inability of a DNA polymerase to extend a mispair directly affects the establishment of genomic mutations. We report here kinetic analyses of the ability of Dpo4, a Y-family polymerase from Sulfolobus solfataricus, to extend from all mispairs opposite a template G or T. Dpo4 is equally inefficient at extending these mispairs, which include, surprisingly, a G.T mispair expected to conform closely to Watson-Crick geometry. To elucidate the basis of this, we solved the structure of Dpo4 bound to G.T-mispaired primer template in the presence of an incoming nucleotide. As a control, we also determined the structure of Dpo4 bound to a matched A-T base pair at the primer terminus. The structures offer a basis for the low efficiency of Dpo4 in extending a G.T mispair: a reverse wobble that deflects the primer 3'-OH away from the incoming nucleotide.

PubMed: 15077104
DOI: 10.1038/nsmb755

実験手法

X-RAY DIFFRACTION (2.4 Å)