1S4X

NMR Structure of the integrin B3 cytoplasmic domain in DPC micelles

1S4X の概要

• エントリーDOI: 10.2210/pdb1s4x/pdb
• 関連するPDBエントリー: 1S4W
• 分子名称: Integrin beta-3 (1 entity in total)
• 機能のキーワード: cell adhesion
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P05106
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7757.64

構造登録者

Vinogradova, O.,Vaynberg, J.,Kong, X.,Haas, T.A.,Plow, E.F.,Qin, J. (登録日: 2004-01-19, 公開日: 2004-03-09, 最終更新日: 2024-05-22)

主引用文献

Vinogradova, O.,Vaynberg, J.,Kong, X.,Haas, T.A.,Plow, E.F.,Qin, J.
Membrane-mediated structural transitions at the cytoplasmic face during integrin activation.
Proc.Natl.Acad.Sci.USA, 101:4094-4099, 2004

PubMed Abstract: Cytoplasmic face-mediated integrin inside-out activation remains a paradigm in transmembrane signal transduction. Emerging evidence suggests that this process involves dissociation of the complex between the integrin cytoplasmic tails; however, a dynamic image of how it occurs on the membrane surface remains elusive. We show here that, whereas membrane-proximal helices of integrin alpha/beta cytoplasmic tails associate in cytoplasm-like aqueous medium, they become partially embedded into membrane-mimetic micelles when unclasped. Membrane embedding induces substantial structural changes of the cytoplasmic tails as compared to their aqueous conformations and suggests there may be an upward movement of the membrane-proximal helices into the membrane during their separation. We further demonstrate that the beta3 tail exhibits additional membrane binding site at its C terminus containing the NPLY motif. Talin, a key intracellular integrin activator, recognizes this site as well as the membrane-proximal helix, thereby promoting cytoplasmic tail separation along the membrane surface. These data provide a structural basis of membrane-mediated changes at the cytoplasmic face in regulating integrin activation and signaling.

PubMed: 15024114
DOI: 10.1073/pnas.0400742101

実験手法

SOLUTION NMR