1S4U

Crystal Structure analysis of the beta-propeller protein Ski8p

Summary for 1S4U

• Entry DOI: 10.2210/pdb1s4u/pdb
• Descriptor: Antiviral protein SKI8 (2 entities in total)
• Functional Keywords: wd repeat, antiviral protein
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Cellular location: Cytoplasm: Q02793
• Total number of polymer chains: 1
• Total formula weight: 45222.54

Authors

Cheng, Z.,Song, H. (deposition date: 2004-01-18, release date: 2004-12-28, Last modification date: 2024-03-13)

Primary citation

Cheng, Z.,Liu, Y.,Wang, C.,Parker, R.,Song, H.
Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination
Protein Sci., 13:2673-2684, 2004

PubMed Abstract: Ski8p is a WD-repeat protein with an essential role for the Ski complex assembly in an exosome-dependent 3'-to-5' mRNA decay. In addition, Ski8p is involved in meiotic recombination by interacting with Spo11p protein. We have determined the crystal structure of Ski8p from Saccharomyces cerevisiae at 2.2 A resolution. The structure reveals that Ski8p folds into a seven-bladed beta propeller. Mapping sequence conservation and hydrophobicities of amino acids on the molecular surface of Ski8p reveals a prominent site on the top surface of the beta propeller, which is most likely involved in mediating interactions of Ski8p with Ski3p and Spo11p. Mutagenesis combined with yeast two-hybrid and GST pull-down assays identified the top surface of the beta propeller as being required for Ski8p binding to Ski3p and Spo11p. The functional implications for Ski8p function in both mRNA decay and meiotic recombination are discussed.

PubMed: 15340168
DOI: 10.1110/ps.04856504

Experimental method

X-RAY DIFFRACTION (2.1 Å)