1S49

Crystal Structure of RNA-dependent RNA polymerase construct 1 (residues 71-679) from bovine viral diarrhea virus complexed with GTP

1S49 の概要

• エントリーDOI: 10.2210/pdb1s49/pdb
• 関連するPDBエントリー: 1S48 1S4F
• 分子名称: RNA-dependent RNA polymerase, GUANOSINE-5'-TRIPHOSPHATE (2 entities in total)
• 機能のキーワード: polymerase, rna synthesis, de novo initiation, primer independent initiation, gtp binding, bovine viral diarrhea virus, bvdv, replication, rna binding protein
• 由来する生物種: Bovine viral diarrhea virus 1
• 細胞内の位置: E(rns) glycoprotein: Host membrane; Peripheral membrane protein. Envelope glycoprotein E2: Host cell surface. Cysteine protease NS2: Host membrane; Multi- pass membrane protein (Potential): P19711
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 71013.92

構造登録者

Choi, K.H.,Groarke, J.M.,Young, D.C.,Kuhn, R.J.,Smith, J.L.,Pevear, D.C.,Rossmann, M.G. (登録日: 2004-01-15, 公開日: 2004-04-06, 最終更新日: 2024-10-30)

主引用文献

Choi, K.H.,Groarke, J.M.,Young, D.C.,Kuhn, R.J.,Smith, J.L.,Pevear, D.C.,Rossmann, M.G.
The structure of the RNA-dependent RNA polymerase from bovine viral diarrhea virus establishes the role of GTP in de novo initiation.
Proc.Natl.Acad.Sci.Usa, 101:4425-4430, 2004

PubMed Abstract: The bovine viral diarrhea virus (BVDV) RNA-dependent RNA polymerase can initiate RNA replication by a de novo mechanism without a primer. The structure of BVDV polymerase, determined to 2.9-A resolution, contains a unique N-terminal domain, in addition to the fingers, palm, and thumb domains common to other polymerases. The structure of BVDV polymerase complexed with GTP, which is required for de novo (primer-independent) initiation, shows that GTP binds adjacent to the initiation NTP, suggesting that the GTP mimics a vestigial RNA product. Comparison of five monomers in two different crystal forms showed conformational changes in the fingertip region and in the thumb domain that may help to translocate the RNA template and product strands during elongation. The putative binding sites of previously reported BVDV inhibitors are also discussed.

PubMed: 15070734
DOI: 10.1073/pnas.0400660101

実験手法

X-RAY DIFFRACTION (3 Å)