1S1K

INFLUENCE OF GROOVE INTERACTIONS ON DNA HOLLIDAY JUNCTION FORMATION

1S1K の概要

• エントリーDOI: 10.2210/pdb1s1k/pdb
• 関連するPDBエントリー: 1P4Z
• 分子名称: 5'-D(*CP*CP*(1AP)P*GP*TP*AP*CP*TP*GP*G)-3', CALCIUM ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: b-dna, double helix, 2, 6-diaminopurine, minor groove interactions, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3163.16

構造登録者

Hays, F.A.,Watson, J.,Ho, P.S. (登録日: 2004-01-06, 公開日: 2004-08-31, 最終更新日: 2023-08-23)

主引用文献

Hays, F.A.,Jones, Z.J.,Ho, P.S.
Influence of minor groove substituents on the structure of DNA holliday junctions.
Biochemistry, 43:9813-9822, 2004

PubMed Abstract: The inosine-containing sequence d(CCIGTACm(5)CGG) is shown to crystallize as a four-stranded DNA junction. This structure is nearly identical to the antiparallel junction formed by the parent d(CCGGTACm(5)()CGG) sequence [Vargason, J. M., and Ho, P. S. (2002) J. Biol. Chem. 277, 21041-21049] in terms of its conformational geometry, and inter- and intramolecular interactions within the DNA and between the DNA and solvent, even though the 2-amino group in the minor groove of the important G(3).m(5)C(8) base pair of the junction core trinucleotide (italicized) has been removed. In contrast, the analogous 2,6-diaminopurine sequence d(CCDGTACTGG) crystallizes as resolved duplex DNAs, just like its parent sequence d(CCAGTACTGG) [Hays, F. A., Vargason, J. M., and Ho, P. S. (2003) Biochemistry 42, 9586-9597]. These results demonstrate that it is not the presence or absence of the 2-amino group in the minor groove of the R(3).Y(8) base pair that specifies whether a sequence forms a junction, but the positions of the extracyclic amino and keto groups in the major groove. Finally, the study shows that the arms of the junction can accommodate perturbations to the B-DNA conformation of the stacked duplex arms associated with the loss of the 2-amino substituent, and that two hydrogen bonding interactions from the C(7) and Y(8) pyrimidine nucleotides to phosphate oxygens of the junction crossover specify the geometry of the Holliday junction.

PubMed: 15274635
DOI: 10.1021/bi049461d

実験手法

X-RAY DIFFRACTION (1.9 Å)