1S0U

eIF2gamma apo

1S0U の概要

• エントリーDOI: 10.2210/pdb1s0u/pdb
• 分子名称: Translation initiation factor 2 gamma subunit, ZINC ION (3 entities in total)
• 機能のキーワード: translation initiation, gtpase, ef-1a, trna, eif2, translation
• 由来する生物種: Methanocaldococcus jannaschii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44216.46

構造登録者

Roll-Mecak, A.,Alone, P.,Cao, C.,Dever, T.E.,Burley, S.K. (登録日: 2004-01-04, 公開日: 2004-01-20, 最終更新日: 2024-10-16)

主引用文献

Roll-Mecak, A.,Alone, P.,Cao, C.,Dever, T.E.,Burley, S.K.
X-ray Structure of Translation Initiation Factor eIF2gamma: IMPLICATIONS FOR tRNA AND eIF2alpha BINDING.
J.Biol.Chem., 279:10634-10642, 2004

PubMed Abstract: The x-ray structure of the gamma-subunit of the heterotrimeric translation initiation factor eIF2 has been determined to 2.4-A resolution. eIF2 is a GTPase that delivers the initiator Met-tRNA to the P site on the small ribosomal subunit during a rate-limiting initiation step in translation. The structure of eIF2gamma closely resembles that of EF1A.GTP, consisting of an N-terminal G domain followed by two beta-barrels arranged in a closed configuration with domain II packed against the G domain in the vicinity of the Switch regions. The G domain of eIF2gamma has an unusual zinc ribbon motif, not previously found in other GTPases. Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of eIF2gamma that bind the alpha-subunit and Met-tRNA(i)(Met), respectively. These structural, biochemical, and genetic results provide new insights into eIF2 ternary complex assembly.

PubMed: 14688270
DOI: 10.1074/jbc.M310418200

実験手法

X-RAY DIFFRACTION (2.4 Å)