1RP3

Cocrystal structure of the flagellar sigma/anti-sigma complex, Sigma-28/FlgM

1RP3 の概要

• エントリーDOI: 10.2210/pdb1rp3/pdb
• 分子名称: RNA polymerase sigma factor SIGMA-28 (FliA), anti sigma factor FlgM (3 entities in total)
• 機能のキーワード: transcription, sigma factor
• 由来する生物種: Aquifex aeolicus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 151971.44

構造登録者

Sorenson, M.K.,Ray, S.S.,Darst, S.A. (登録日: 2003-12-02, 公開日: 2004-04-06, 最終更新日: 2024-02-14)

主引用文献

Sorenson, M.K.,Ray, S.S.,Darst, S.A.
Crystal Structure of the Flagellar Sigma/Anti-Sigma Complex Sigma(28)/FlgM Reveals an Intact Sigma Factor in an Inactive Conformation
Mol.Cell, 14:127-138, 2004

PubMed Abstract: The key regulators of bacterial transcription initiation are the sigma factors, which direct promoter recognition and melting but only after binding to the core RNA polymerase to form the holoenzyme. X-ray crystal structures of the flagellar sigma, sigma(28), in complex with its anti-sigma, FlgM, explain the inhibition mechanism of FlgM, including its ability to attack and destabilize the sigma(28)-holoenzyme. The sigma domains (sigma(2), sigma(3), and sigma(4)) pack together in a compact unit with extensive interdomain interfaces that bury the promoter binding determinants, including the -35 element recognition helix of sigma(4), which fits in an acidic groove on the surface of sigma(3). The structure illustrates the large rearrangements that sigma(28) must undergo to form the holoenzyme and provides insights into the regulation of sigma(28) promoter binding activity that may extend, at least in principle, to other sigmas.

PubMed: 15068809
DOI: 10.1016/S1097-2765(04)00150-9

実験手法

X-RAY DIFFRACTION (2.3 Å)