1ROM

CRYSTAL STRUCTURE OF NITRIC REDUCTASE FROM DENITRIFYING FUNGUS FUSARIUM OXYSPORUM

1ROM の概要

• エントリーDOI: 10.2210/pdb1rom/pdb
• 分子名称: CYTOCHROME P450, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: nitric oxide reductase, cytochrome p450nor, oxidoreductase
• 由来する生物種: Fusarium oxysporum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45037.18

構造登録者

Park, S.-Y.,Nakagawa, A. (登録日: 1997-03-24, 公開日: 1997-10-15, 最終更新日: 2024-02-14)

主引用文献

Park, S.Y.,Shimizu, H.,Adachi, S.,Nakagawa, A.,Tanaka, I.,Nakahara, K.,Shoun, H.,Obayashi, E.,Nakamura, H.,Iizuka, T.,Shiro, Y.
Crystal structure of nitric oxide reductase from denitrifying fungus Fusarium oxysporum.
Nat.Struct.Biol., 4:827-832, 1997

PubMed Abstract: Structures of nitric oxide reductase (NOR) in the ferric resting and the ferrous CO states have been solved at 2.0 A resolution. These structures provide significant new insights into how NO is reduced in biological systems. The haem distal pocket is open to solvent, implicating this region as a possible NADH binding site. In combination with mutagenesis results, a hydrogen-bonding network from the water molecule adjacent to the iron ligand to the protein surface of the distal pocket through the hydroxyl group of Ser 286 and the carboxyl group of Asp 393 can be assigned to a pathway for proton delivery during the NO reduction reaction.

PubMed: 9334748
DOI: 10.1038/nsb1097-827

実験手法

X-RAY DIFFRACTION (2 Å)