1RMR

Crystal Structure of Schistatin, a Disintegrin Homodimer from saw-scaled Viper (Echis carinatus) at 2.5 A resolution

1RMR の概要

• エントリーDOI: 10.2210/pdb1rmr/pdb
• 分子名称: Disintegrin schistatin (2 entities in total)
• 機能のキーワード: disintegrin, homodimer, echis carinatus, protein binding
• 由来する生物種: Echis carinatus (saw-scaled viper)
• 細胞内の位置: Secreted: P83658
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7090.94

構造登録者

Bilgrami, S.,Tomar, S.,Yadav, S.,Kaur, P.,Kumar, J.,Jabeen, T.,Sharma, S.,Singh, T.P. (登録日: 2003-11-28, 公開日: 2004-06-16, 最終更新日: 2024-10-09)

主引用文献

Bilgrami, S.,Tomar, S.,Yadav, S.,Kaur, P.,Kumar, J.,Jabeen, T.,Sharma, S.,Singh, T.P.
Crystal structure of schistatin, a disintegrin homodimer from saw-scaled viper (Echis carinatus) at 2.5 A resolution
J.Mol.Biol., 341:829-837, 2004

PubMed Abstract: This is the first structure of a biological homodimer of disintegrin. Disintegrins are a class of small (4-14 kDa) proteins that bind to transmembrane integrins selectively. The present molecule is the first homodimer that has been isolated from the venom of Echis carinatus. The monomeric chain contains 64 amino acid residues. The three-dimensional structure of schistatin has been determined by the multiple isomorphous replacement method. It has been refined to an R-factor of 0.190 using all the data to 2.5 A resolution. The two subunits of the disintegrin homodimer are related by a 2-fold crystallographic symmetry. Thus, the crystallographic asymmetric unit contains a monomer of disintegrin. The monomer folds into an up-down topology with three sets of antiparallel beta-strands. The structure is well ordered with four intramolecular disulfide bonds. the two monomers are firmly linked to each other through two intermolecular disulfide bridges at their N termini together with several other interactions. This structure has corrected the error in the disulfide bond pattern of the two intermolecular disulfide bridges that was reported earlier using chemical methods. Unique sequence and structural features of the schistatin monomers suggest that they have the ability to bind well with both alphaIIb beta3 and alphav beta3 integrins. The N termini anchored two chains of the dimer diverge away at their C termini exposing the Arg-Gly-Asp motif into opposite directions thus enhancing their binding efficiency to integrins. This is one of the unique features of the present disintegrin homodimer and seems to be responsible for the clustering of integrin molecules. The homodimer binds to integrins apparently with a higher affinity than the monomers and also plays a role in the signaling pathway.

PubMed: 15317139
DOI: 10.1016/j.jmb.2004.06.048

実験手法

X-RAY DIFFRACTION (2.5 Å)