1RI0

NMR structure of the N-terminal hath domain of human HDGF

1RI0 の概要

• エントリーDOI: 10.2210/pdb1ri0/pdb
• 関連するPDBエントリー: 1H3Z 1KHC
• NMR情報: BMRB: 5902
• 分子名称: Hepatoma-derived growth factor (1 entity in total)
• 機能のキーワード: hdgf, hath domain, pwwp domain, heparin-binding, growth factor, hormone-growth factor complex, hormone/growth factor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P51858
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12797.55

構造登録者

Sue, S.-C.,Chen, J.-Y.,Huang, T.-H. (登録日: 2003-11-16, 公開日: 2004-11-16, 最終更新日: 2024-05-29)

主引用文献

Sue, S.-C.,Chen, J.-Y.,Lee, S.-C.,Wu, W.-G.,Huang, T.-H.
Solution Structure and Heparin Interaction of Human Hepatoma-derived Growth Factor
J.Mol.Biol., 343:1365-1377, 2004

PubMed Abstract: Hepatoma-derived growth factor (HDGF)-related proteins (HRPs) comprise a new protein family that has been implicated in nephrogenesis, tumorigenesis, vascular development, cell proliferation, and transcriptional activation. All HRPs share a conserved N-terminal homologous to the amino terminus of HDGF (HATH) domain, but vary significantly in the C-terminal region. Here, we show that in solution the N and C termini of human HDGF form two structurally independent domains. The 100 amino acid residue N-terminal HATH domain is well-structured while the 140 amino acid residue C-terminal domain is disordered. We determined the solution structure of the HATH domain by NMR. The core structure of the HATH domain is a five-stranded beta-barrel followed by two alpha-helices, similar to those of PWWP domains of known structures. Surface plasmon resonance results showed that the HATH domain is primarily responsible for heparin binding. On the basis of the chemical shift perturbation induced by binding of heparin-derived hexasaccharide, we identified a prominent, highly positively charged region as the putative heparin-binding site. Sequence comparison and structure prediction suggest that all HRPs are likely to adapt a similar modular structure.

PubMed: 15491618
DOI: 10.1016/j.jmb.2004.09.014

実験手法

SOLUTION NMR