1RGC

THE COMPLEX BETWEEN RIBONUCLEASE T1 AND 3'-GUANYLIC ACID SUGGESTS GEOMETRY OF ENZYMATIC REACTION PATH. AN X-RAY STUDY

1RGC の概要

• エントリーDOI: 10.2210/pdb1rgc/pdb
• 分子名称: RIBONUCLEASE T1, CALCIUM ION, GUANOSINE-3'-MONOPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: hydrolase(endoribonuclease)
• 由来する生物種: Aspergillus oryzae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22995.99

構造登録者

Heydenreich, A.,Koellner, G.,Choe, H.W.,Cordes, F.,Kisker, C.,Schindelin, H.,Adamiak, R.,Hahn, U.,Saenger, W. (登録日: 1993-05-12, 公開日: 1994-01-31, 最終更新日: 2024-10-30)

主引用文献

Heydenreich, A.,Koellner, G.,Choe, H.W.,Cordes, F.,Kisker, C.,Schindelin, H.,Adamiak, R.,Hahn, U.,Saenger, W.
The complex between ribonuclease T1 and 3'GMP suggests geometry of enzymic reaction path. An X-ray study.
Eur.J.Biochem., 218:1005-1012, 1993

PubMed Abstract: The crystal structure of the complex between ribonuclease T1 and 3'GMP suggests that (a) a substrate GpN is bound to the active site of ribonuclease T1 in a conformation that actively supports the catalytic process, (b) the reaction occurs in an in-line process, (c) His40 N epsilon H+ activates O2'-H, (d) Glu58 carboxylate acts as base and His92 N epsilon H+ as acid in a general acid-base catalysis. The crystals have the monoclinic space group P2(1), a = 4.968 nm, b = 4.833 nm, c = 4.048 nm, beta = 90.62 degrees with two molecules in the asymmetric unit. The structure was determined by molecular replacement and refined to R = 15.3% with 11,338 data > or = 1 sigma (Fo) in the resolution range 1.0-0.2 nm; this includes 180 water molecules and two Ca2+. The structure of ribonuclease T1 is as previously observed. 3'GMP is bound in syn conformation; guanine is located in the specific recognition site, the ribose adopts C4'-exo puckering, the ribose phosphate is extended with torsion angle epsilon in trans. The O2'-H group is activated by accepting and donating hydrogen bonds from His40 N epsilon H+ and to Glu58 O epsilon 1; the phosphate is hydrogen bonded to Glu58 O epsilon 2H, Arg77 N epsilon H+ and N eta 2H+, Tyr38 O eta H, His92 N eta H+. The conformation of ribose phosphate is such that O2' is at a distance of 0.31 nm from phosphorus, and opposite the P-OP3 bond which accepts a hydrogen bond from His92 N epsilon H+; we infer from a model building study that this bond is equivalent to the scissile P-O5' in a substrate GpN.

PubMed: 8281918
DOI: 10.1111/j.1432-1033.1993.tb18459.x

実験手法

X-RAY DIFFRACTION (2 Å)