1RFQ

Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer

1RFQ の概要

• エントリーDOI: 10.2210/pdb1rfq/pdb
• 関連するPDBエントリー: 1RDW
• 分子名称: Actin, alpha skeletal muscle, MAGNESIUM ION, ADENOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: anti-parallel dimer, polymerization, nucleation, filament, structural protein
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• 細胞内の位置: Cytoplasm, cytoskeleton: P68135
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 85631.30

構造登録者

Reutzel, R.,Yoshioka, C.,Govindasamy, L.,Yarmola, E.G.,Agbandje-McKenna, M.,Bubb, M.R.,McKenna, R. (登録日: 2003-11-10, 公開日: 2003-12-16, 最終更新日: 2025-11-12)

主引用文献

Reutzel, R.,Yoshioka, C.,Govindasamy, L.,Yarmola, E.G.,Agbandje-McKenna, M.,Bubb, M.R.,McKenna, R.
Actin crystal dynamics: structural implications for F-actin nucleation, polymerization, and branching mediated by the anti-parallel dimer.
J.Struct.Biol., 146:291-301, 2004

PubMed Abstract: Actin filament nucleation, polymerization, and branching are crucial steps in many forms of cell motility, cell shape, and intracellular organelle movements in a wide range of organisms. Previous biochemical data suggests that an anti-parallel actin dimer can incorporate itself into growing filamentous actin (F-actin) and has a role in branching. Furthermore, it is a widespread belief that nucleation is spawned from an actin trimer complex. Here we present the structures of actin dimers and trimers in two tetragonal crystal systems P4(3)2(1)2 and P4(3). Both crystal systems formed by an induced condensation transformation of a previously reported orthorhombic crystal system P2(1)2(1)2(1). Comparison between the three crystal systems demonstrates the dynamics and flexibility of actin-actin interactions. The dimer and trimer actin rearrangements observed between the three crystal systems may provide insight to in vivo actin-actin interactions that occur during the nucleation, polymerization, and branching of F-actin.

PubMed: 15099571
DOI: 10.1016/j.jsb.2003.12.006

実験手法

X-RAY DIFFRACTION (3 Å)