1RFG

Crystal Structure of Human Purine Nucleoside Phosphorylase Complexed with Guanosine

1RFG の概要

• エントリーDOI: 10.2210/pdb1rfg/pdb
• 分子名称: Purine nucleoside phosphorylase, SULFATE ION, GUANOSINE, ... (4 entities in total)
• 機能のキーワード: purine nucleoside phosphorylase, drug design, synchrotron, guanosine, transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, cytoskeleton (By similarity): P00491
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32625.11

構造登録者

Canduri, F.,Silva, R.G.,Dos Santos, D.M.,Palma, M.S.,Basso, L.A.,Santos, D.S.,de Azevedo, W.F. (登録日: 2003-11-09, 公開日: 2004-12-14, 最終更新日: 2023-08-23)

主引用文献

Canduri, F.,Silva, R.G.,dos Santos, D.M.,Palma, M.S.,Basso, L.A.,Santos, D.S.,de Azevedo, W.F.
Structure of human PNP complexed with ligands.
Acta Crystallogr.,Sect.D, 61:856-862, 2005

PubMed Abstract: Purine nucleoside phosphorylase (PNP) is a key enzyme in the purine-salvage pathway, which allows cells to utilize preformed bases and nucleosides in order to synthesize nucleotides. PNP is specific for purine nucleosides in the beta-configuration and exhibits a strong preference for purines containing a 6-keto group and ribosyl-containing nucleosides relative to the corresponding analogues. PNP was crystallized in complex with ligands and data collection was performed using synchrotron radiation. This work reports the structure of human PNP in complex with guanosine (at 2.80 A resolution), 3'-deoxyguanosine (at 2.86 A resolution) and 8-azaguanine (at 2.85 A resolution). These structures were compared with the PNP-guanine, PNP-inosine and PNP-immucillin-H complexes solved previously.

PubMed: 15983407
DOI: 10.1107/S0907444905005421

実験手法

X-RAY DIFFRACTION (2.9 Å)