1RFB

CRYSTAL STRUCTURE OF RECOMBINANT BOVINE INTERFERON-GAMMA AT 3.0 ANGSTROMS RESOLUTION

1RFB の概要

• エントリーDOI: 10.2210/pdb1rfb/pdb
• 分子名称: INTERFERON-GAMMA (2 entities in total)
• 機能のキーワード: glycoprotein
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted: P07353
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28110.16

構造登録者

Samudzi, C.T.,Rubin, J.R. (登録日: 1993-06-30, 公開日: 1994-01-31, 最終更新日: 2024-02-14)

主引用文献

Samudzi, C.T.,Rubin, J.R.
Structure of recombinant bovine interferon-gamma at 3.0 A resolution.
Acta Crystallogr.,Sect.D, 49:513-521, 1993

PubMed Abstract: The three-dimensional crystal structure of recombinant bovine interferon-gamma was determined using the multiple isomorphous replacement method at 3.0 A and refined to an R factor of 19.2%. This protein crystallizes in space group P2(1)2(1)2(1) with unit-cell parameters of a = 42.8, b = 79.9 and c = 85.4 A. There is one functional dimer in the asymmetric unit. The two polypeptide chains are related by a non-crystallographic twofold symmetry axis. The secondary structure is predominantly alpha-helical with extensive interdigitation of the alpha-helical segments of the polypeptide chains that make up the dimer. The secondary structure, tertiary structure and topology of this molecule are identical to the previously reported structures of recombinant rabbit interferon-gamma and recombinant human interferon-gamma. The molecular topology is also similar to that of murine interferon-beta. These structural similarities strongly indicate the presence of a unique topological feature (fold) among gamma-interferons from different species, and also among the different classes of interferons.

PubMed: 15299487
DOI: 10.1107/S0907444993006924

実験手法

X-RAY DIFFRACTION (3 Å)