1RF8

Solution structure of the yeast translation initiation factor eIF4E in complex with m7GDP and eIF4GI residues 393 to 490

1RF8 の概要

• エントリーDOI: 10.2210/pdb1rf8/pdb
• 関連するPDBエントリー: 1AP8
• 分子名称: Eukaryotic translation initiation factor 4E, Eukaryotic initiation factor 4F subunit p150, S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate, ... (4 entities in total)
• 機能のキーワード: initiation factor, protein biosynthesis, translation regulation, biosynthetic protein, translation
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Cytoplasm: P39935
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36814.36

構造登録者

Gross, J.D.,Moerke, N.J.,von der Haar, T.,Lugovskoy, A.A.,Sachs, A.B.,McCarthy, J.E.G.,Wagner, G. (登録日: 2003-11-07, 公開日: 2003-12-23, 最終更新日: 2024-11-13)

主引用文献

Gross, J.D.,Moerke, N.J.,von der Haar, T.,Lugovskoy, A.A.,Sachs, A.B.,McCarthy, J.E.,Wagner, G.
Ribosome loading onto the mRNA cap is driven by conformational coupling between eIF4G and eIF4E.
Cell(Cambridge,Mass.), 115:739-750, 2003

PubMed Abstract: The eukaryotic initiation factor 4G (eIF4G) is the core of a multicomponent switch controlling gene expression at the level of translation initiation. It interacts with the small ribosomal subunit interacting protein, eIF3, and the eIF4E/cap-mRNA complex in order to load the ribosome onto mRNA during cap-dependent translation. We describe the solution structure of the complex between yeast eIF4E/cap and eIF4G (393-490). Binding triggers a coupled folding transition of eIF4G (393-490) and the eIF4E N terminus resulting in a molecular bracelet whereby eIF4G (393-490) forms a right-handed helical ring that wraps around the N terminus of eIF4E. Cofolding allosterically enhances association of eIF4E with the cap and is required for maintenance of optimal growth and polysome distributions in vivo. Our data explain how mRNA, eIF4E, and eIF4G exists as a stable mRNP that may facilitate multiple rounds of ribosomal loading during translation initiation, a key determinant in the overall rate of protein synthesis.

PubMed: 14675538
DOI: 10.1016/S0092-8674(03)00975-9

実験手法

SOLUTION NMR