1REP

CRYSTAL STRUCTURE OF REPLICATION INITIATOR PROTEIN REPE54 OF MINI-F PLASMID COMPLEXED WITH AN ITERON DNA

Summary for 1REP

• Entry DOI: 10.2210/pdb1rep/pdb
• Descriptor: DNA (5'-D(*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*T)-3'), DNA (5'-D(*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*T)-3'), PROTEIN (REPLICATION INITIATION PROTEIN), ... (5 entities in total)
• Functional Keywords: replication initiator, dna-binding, replication-dna complex, replication/dna
• Biological source: Escherichia coli
• Total number of polymer chains: 3
• Total formula weight: 42862.56

Authors

Komori, H.,Matsunaga, F.,Higuchi, Y.,Ishiai, M.,Wada, C.,Miki, K. (deposition date: 1999-04-29, release date: 2000-02-09, Last modification date: 2023-12-27)

Primary citation

Komori, H.,Matsunaga, F.,Higuchi, Y.,Ishiai, M.,Wada, C.,Miki, K.
Crystal structure of a prokaryotic replication initiator protein bound to DNA at 2.6 A resolution.
EMBO J., 18:4597-4607, 1999

PubMed Abstract: The initiator protein (RepE) of F factor, a plasmid involved in sexual conjugation in Escherichia coli, has dual functions during the initiation of DNA replication which are determined by whether it exists as a dimer or as a monomer. A RepE monomer functions as a replication initiator, but a RepE dimer functions as an autogenous repressor. We have solved the crystal structure of the RepE monomer bound to an iteron DNA sequence of the replication origin of plasmid F. The RepE monomer consists of topologically similar N- and C-terminal domains related to each other by internal pseudo 2-fold symmetry, despite the lack of amino acid similarities between the domains. Both domains bind to the two major grooves of the iteron (19 bp) with different binding affinities. The C-terminal domain plays the leading role in this binding, while the N-terminal domain has an additional role in RepE dimerization. The structure also suggests that superhelical DNA induced at the origin of plasmid F by four RepEs and one HU dimer has an essential role in the initiation of DNA replication.

PubMed: 10469640
DOI: 10.1093/emboj/18.17.4597

Experimental method

X-RAY DIFFRACTION (2.6 Å)