1RB5

ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TRIGONAL FORM

1RB5 の概要

• エントリーDOI: 10.2210/pdb1rb5/pdb
• 関連するPDBエントリー: 1rb1 1rb4 1rb6
• 分子名称: General control protein GCN4 (2 entities in total)
• 機能のキーワード: coiled coil, leucine zipper, automation, dna binding protein
• 細胞内の位置: Nucleus: P03069
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 11966.03

構造登録者

Holton, J.,Alber, T. (登録日: 2003-11-01, 公開日: 2004-01-13, 最終更新日: 2024-10-09)

主引用文献

Holton, J.,Alber, T.
Automated protein crystal structure determination using ELVES.
Proc.Natl.Acad.Sci.USA, 101:1537-1542, 2004

PubMed Abstract: Efficient determination of protein crystal structures requires automated x-ray data analysis. Here, we describe the expert system ELVES and its use to determine automatically the structure of a 12-kDa protein. Multiwavelength anomalous diffraction analysis of a selenomethionyl derivative was used to image the Asn-16-Ala variant of the GCN4 leucine zipper. In contrast to the parallel, dimeric coiled coil formed by the WT sequence, the mutant unexpectedly formed an antiparallel trimer. This structural switch reveals how avoidance of core cavities at a single site can select the native fold of a protein. All structure calculations, including indexing, data processing, locating heavy atoms, phasing by multiwavelength anomalous diffraction, model building, and refinement, were completed without human intervention. The results demonstrate the feasibility of automated methods for determining high-resolution, x-ray crystal structures of proteins.

PubMed: 14752198
DOI: 10.1073/pnas.0306241101

実験手法

X-RAY DIFFRACTION (1.9 Å)