1R8O

Crystal structure of an unusual Kunitz-type trypsin inhibitor from Copaifera langsdorffii seeds

1R8O の概要

• エントリーDOI: 10.2210/pdb1r8o/pdb
• 関連するPDBエントリー: 1R8N
• 分子名称: Kunitz trypsin inhibitor (3 entities in total)
• 機能のキーワード: kunitz (sti) trypsin inhibitor, beta-trefoil fold, copaifera langsdorffii, hydrolase inhibitor
• 由来する生物種: Copaifera langsdorffii; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18031.78

構造登録者

Krauchenco, S.,Nagem, R.A.P.,da Silva, J.A.,Marangoni, S.,Polikarpov, I. (登録日: 2003-10-27, 公開日: 2004-05-25, 最終更新日: 2024-10-30)

主引用文献

Krauchenco, S.,Nagem, R.A.P.,Da Silva, J.A.,Marangoni, S.,Polikarpov, I.
Three-dimensional structure of an unusual Kunitz (STI) type trypsin inhibitor from Copaifera langsdorffii.
Biochimie, 86:167-172, 2004

PubMed Abstract: The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI is the first structure of a member of Kunitz (STI) family formed by two noncovalently bound polypeptide chains and only one disulfide bridge. A standard Kunitz-type inhibitor has a single polypeptide chain and two disulfide bridges. Structural features granting CTI high inhibitory activity are discussed.

PubMed: 15134830
DOI: 10.1016/j.biochi.2004.03.004

実験手法

X-RAY DIFFRACTION (1.83 Å)