1R3M

Crystal structure of the dimeric unswapped form of bovine seminal ribonuclease

Summary for 1R3M

• Entry DOI: 10.2210/pdb1r3m/pdb
• Related: 1BSR 1R5C 1R5D
• Descriptor: Ribonuclease, seminal, PHOSPHATE ION (3 entities in total)
• Functional Keywords: ribonuclease, swapping, hinge, hydrolase
• Biological source: Bos taurus (cattle)
• Cellular location: Secreted: P00669
• Total number of polymer chains: 2
• Total formula weight: 27455.22

Authors

Berisio, R.,Sica, F.,De Lorenzo, C.,Di Fiore, A.,Piccoli, R.,Zagari, A.,Mazzarella, L. (deposition date: 2003-10-02, release date: 2003-11-18, Last modification date: 2024-11-13)

Primary citation

Berisio, R.,Sica, F.,De Lorenzo, C.,Di Fiore, A.,Piccoli, R.,Zagari, A.,Mazzarella, L.
Crystal structure of the dimeric unswapped form of bovine seminal ribonuclease
Febs Lett., 554:105-110, 2003

PubMed Abstract: Bovine seminal ribonuclease is a unique case of protein dimorphism, since it exists in two dimeric forms, with different biological and kinetic behavior, which interconvert into one another through three-dimensional swapping. Here we report the crystal structure, at 2.2 A resolution, of the unswapped form of bovine seminal ribonuclease. Besides completing the structural definition of bovine seminal ribonuclease conformational dimorphism, this study provides the structural basis to explain the dependence of the enzyme cooperative effects on its swapping state.

PubMed: 14596923
DOI: 10.1016/S0014-5793(03)01114-1

Experimental method

X-RAY DIFFRACTION (2.2 Å)