1R3H

Crystal Structure of T10

Summary for 1R3H

• Entry DOI: 10.2210/pdb1r3h/pdb
• Related: 1c16
• Descriptor: MHC H2-TL-T10-129, Beta-2-microglobulin (2 entities in total)
• Functional Keywords: class ib mhc, gammadelta t cell ligand, immune system
• Biological source: Mus musculus (house mouse); More
• Cellular location: Secreted: P61769
• Total number of polymer chains: 8
• Total formula weight: 166762.08

Authors

Rudolph, M.G.,Wilson, I.A. (deposition date: 2003-10-02, release date: 2004-03-30, Last modification date: 2024-11-13)

Primary citation

Rudolph, M.G.,Wingren, C.,Crowley, M.P.,Chien, Y.H.,Wilson, I.A.
Combined pseudo-merohedral twinning, non-crystallographic symmetry and pseudo-translation in a monoclinic crystal form of the gammadelta T-cell ligand T10.
Acta Crystallogr.,Sect.D, 60:656-664, 2004

PubMed Abstract: T10 is a non-classical class Ib-like major histocompatibility complex (MHC) cell-surface antigen which binds directly to certain gammadelta T-cell receptors in the absence of any exogenous and endogenous ligands, such as peculiar lipids or glycolipids. The crystal structure at 2.5 A resolution of murine T10 was determined by molecular replacement using data from an almost perfectly twinned monoclinic crystal. The space group is P2(1), with unit-cell parameters a = 78.2, b = 70.0, c = 139.2 A, beta = 106.8 degrees. Self-rotation function analysis and various intensity statistics revealed the presence of pseudo-merohedral twinning, but these tests underestimated the true twin fraction of alpha approximately 0.46. Native Patterson analyses pointed to the presence of pseudo-translation among the four molecules present in the asymmetric unit. Data analysis, structure determination and model refinement are discussed.

PubMed: 15039553
DOI: 10.1107/S0907444904002239

Experimental method

X-RAY DIFFRACTION (2.5 Å)