1QYY

Crystal Structure of N-Terminal Domain of Human Platelet Receptor Glycoprotein Ib-alpha at 2.8 Angstrom Resolution

1QYY の概要

• エントリーDOI: 10.2210/pdb1qyy/pdb
• 関連するPDBエントリー: 1OOK 1P9A
• 分子名称: Platelet glycoprotein Ib alpha chain, alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, PLATINUM (II) ION, ... (5 entities in total)
• 機能のキーワード: platelet receptors, glycocalicin, leucine rich repeats, cell adhesion
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P07359
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66168.70

構造登録者

Varughese, K.I.,Ruggeri, Z.M.,Celikel, R. (登録日: 2003-09-12, 公開日: 2004-03-02, 最終更新日: 2024-11-13)

主引用文献

Varughese, K.I.,Ruggeri, Z.M.,Celikel, R.
Platinum-induced space-group transformation in crystals of the platelet glycoprotein Ib alpha N-terminal domain.
Acta Crystallogr.,Sect.D, 60:405-411, 2004

PubMed Abstract: The interaction between platelet glycoprotein (GP) Ib alpha and von Willebrand factor (VWF) is essential for thrombus formation, leading to the arrest of bleeding. The N-terminal domain of GP Ib alpha, which contains the binding sites for VWF and alpha-thrombin, crystallized in the tetragonal space group P4(3) with one molecule in the asymmetric unit. When the crystals were treated with platinum, the crystals changed their symmetry from tetragonal to monoclinic P2(1) with two molecules in the asymmetric unit. The structure of the monoclinic form was solved using two-wavelength platinum anomalous dispersion data. The tetragonal crystal structure was subsequently solved using molecular-replacement techniques using the monoclinic structure as the search model and was refined with 1.7 A resolution data.

PubMed: 14993663
DOI: 10.1107/S0907444903026805

実験手法

X-RAY DIFFRACTION (2.8 Å)