1QUZ

Solution structure of the potassium channel scorpion toxin HSTX1

1QUZ の概要

• エントリーDOI: 10.2210/pdb1quz/pdb
• NMR情報: BMRB: 4541
• 分子名称: HSTX1 TOXIN (1 entity in total)
• 機能のキーワード: scorpion toxin, alpha beta, molecular modeling, potassium channel, toxin
• 細胞内の位置: Secreted: P59867
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3834.59

構造登録者

Savarin, P.,Romi-Lebrun, R.,Zinn-Justin, S.,Lebrun, B.,Nakajima, T.,Gilquin, B.,Menez, A. (登録日: 1999-07-05, 公開日: 2000-07-07, 最終更新日: 2022-03-02)

主引用文献

Savarin, P.,Romi-Lebrun, R.,Zinn-Justin, S.,Lebrun, B.,Nakajima, T.,Gilquin, B.,Menez, A.
Structural and functional consequences of the presence of a fourth disulfide bridge in the scorpion short toxins: solution structure of the potassium channel inhibitor HsTX1.
Protein Sci., 8:2672-2685, 1999

PubMed Abstract: We have determined the three-dimensional structure of the potassium channel inhibitor HsTX1, using nuclear magnetic resonance and molecular modeling. This protein belongs to the scorpion short toxin family, which essentially contains potassium channel blockers of 29 to 39 amino acids and three disulfide bridges. It is highly active on voltage-gated Kv1.3 potassium channels. Furthermore, it has the particularity to possess a fourth disulfide bridge. We show that HsTX1 has a fold similar to that of the three-disulfide-bridged toxins and conserves the hydrophobic core found in the scorpion short toxins. Thus, the fourth bridge has no influence on the global conformation of HsTX1. Most residues spatially analogous to those interacting with voltage-gated potassium channels in the three-disulfide-bridged toxins are conserved in HsTX1. Thus, we propose that Tyr21, Lys23, Met25, and Asn26 are involved in the biological activity of HsTX1. As an additional positively charged residue is always spatially close to the aromatic residue in toxins blocking the voltage-gated potassium channels, and as previous mutagenesis experiments have shown the critical role played by the C-terminus in HsTX1, we suggest that Arg33 is also important for the activity of the four disulfide-bridged toxin. Docking calculations confirm that, if Lys23 and Met25 interact with the GYGDMH motif of Kv1.3, Arg33 can contact Asp386 and, thus, play the role of the additional positively charged residue of the toxin functional site. This original configuration of the binding site of HsTX1 for Kv1.3, if confirmed experimentally, offers new structural possibilities for the construction of a molecule blocking the voltage-gated potassium channels.

PubMed: 10631983

実験手法

SOLUTION NMR