1QPW

CRYSTAL STRUCTURE DETERMINATION OF PORCINE HEMOGLOBIN AT 1.8A RESOLUTION

1QPW の概要

• エントリーDOI: 10.2210/pdb1qpw/pdb
• 分子名称: PORCINE HEMOGLOBIN (ALPHA SUBUNIT), PORCINE HEMOGLOBIN (BETA SUBUNIT), PROTOPORPHYRIN IX CONTAINING FE, ... (5 entities in total)
• 機能のキーワード: x-ray study, porcine hemoglobin, artificial human blood, oxygen transport
• 由来する生物種: Sus scrofa (pig); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64776.92

構造登録者

Lu, T.-H.,Panneerselvam, K.,Liaw, Y.-C.,Kan, P.,Lee, C.-J. (登録日: 1999-05-30, 公開日: 1999-06-04, 最終更新日: 2024-02-14)

主引用文献

Lu, T.H.,Panneerselvam, K.,Liaw, Y.C.,Kan, P.,Lee, C.J.
Structure determination of porcine haemoglobin.
Acta Crystallogr.,Sect.D, 56:304-312, 2000

PubMed Abstract: To investigate a potential candidate material for making artificial red blood cells to supplement blood transfusion, the X-ray structure of porcine haemoglobin at 1.8 A resolution was determined as part of research towards synthesizing human blood. Porcine haemoglobin was crystallized by the vapor-diffusion method, producing crystals of dimensions 0.3-0.5 mm after successive seeding. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 68.10, b = 72.27, c = 114.85 A. The initial phase was determined by the molecular-replacement method, using human oxyhaemoglobin as a model. The final R factor was 21.1% for 36 820 reflections after validation of 574 water molecules. The r.m.s. deviations of bond lengths, angles, torsion angles and improper angles from their ideal values are 0.017 A, 3.0, 20.6 and 1.8 degrees, respectively. The average B factor is 33.63 A(2) for the haemoglobin molecule and 50.53 A(2) for the water molecules. The structure could be superimposed on a 2.8 A resolution structure with an r.m.s. difference of 0.59 A in main-chain atomic positions and 1. 27 A in side-chain atomic positions. Porcine and human haemoglobins are compared. A tentative model for artificial blood is proposed based on the complementarity relationship of the surface charges between haemoglobin and the surrounding cell membrane.

PubMed: 10713517
DOI: 10.1107/S0907444900000093

実験手法

X-RAY DIFFRACTION (1.8 Å)