1QNO

The 3-D structure of a Trichoderma reesei b-mannanase from glycoside hydrolase family 5

1QNO の概要

• エントリーDOI: 10.2210/pdb1qno/pdb
• 関連するPDBエントリー: 1QNP 1QNQ 1QNR 1QNS
• 分子名称: ENDO-1,4-B-D-MANNANASE, 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total)
• 機能のキーワード: hydrolase, mannanase, trichoderma reesei, anomalous scattering
• 由来する生物種: TRICHODERMA REESEI
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38754.11

構造登録者

Sabini, E.,Schubert, H.,Murshudov, G.,Wilson, K.S.,Siika-Aho, M.,Penttila, M. (登録日: 1999-10-20, 公開日: 2000-10-22, 最終更新日: 2024-11-13)

主引用文献

Sabini, E.,Schubert, H.,Murshudov, G.,Wilson, K.S.,Siika-Aho, M.,Penttila, M.
The Three-Dimensional Structure of a Trichoderma Reesei Beta-Mannanase from Glycoside Hydrolase Family 5.
Acta Crystallogr.,Sect.D, 56:3-, 2000

PubMed Abstract: The crystal structure of the catalytic core domain of beta-mannanase from the fungus Trichoderma reesei has been determined at a resolution of 1.5 A. The structure was solved using the anomalous scattering from a single non-isomorphous platinum complex with two heavy-metal sites in space group P2(1). The map computed with the experimental phases was enhanced by the application of an automated model building and refinement procedure using the amplitudes and experimental phases as observations. This approach is expected to be of more general application. The structure of the native enzyme and complexes with Tris-HCl and mannobiose are also reported: the mannobiose binds in subsites +1 and +2. The structure is briefly compared with that of the homologous beta-mannanase from the bacterium Thermomonospora fusca.

PubMed: 10666621
DOI: 10.1107/S0907444999013943

実験手法

X-RAY DIFFRACTION (2 Å)